Research Scientist I · Charles River Laboratories

Nadia Tasnim Ahmed PhD

Analytical Scientist — Bioanalytical Method Development
// Charles River Laboratories · Ashland, Ohio

PhD in Pharmaceutical Engineering with 5+ years in LC-MS/MS and high-resolution mass spectrometry. Expert in bioanalytical method development and validation for small molecules, peptides, proteins, and oligonucleotides across regulated GLP environments. Former ORISE Fellow at the FDA/CDER.

LC-MS/MS Bioanalytical Methods Oligonucleotide Analysis Proteomics Ion Mobility-MS GLP / GxP FDA Regulatory
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Nadia Tasnim Ahmed
Nadia Tasnim Ahmed, PhD
Research Scientist I · Charles River Laboratories
8+Publications
7Conf. Talks
12+Client Projects
5+Yrs MS Exp.
Research Interests
LC-MS/MS Bioanalytical Methods
Oligonucleotide Characterization
Proteomics & PTM Analysis
Ion Mobility Mass Spectrometry
Regulatory Method Validation
Experience

Career timeline

Jun 2025 – Present Charles River Laboratories Ashland, Ohio Current
Research Scientist I, Method Development
  • Lead regulated LC-MS/MS bioanalytical method development and qualification for small and large molecule programs across plasma, serum, tissue, and whole blood matrices, supporting 12+ client-sponsored projects.
  • Collaborate with study directors, validation teams, and cross-functional stakeholders to design fit-for-purpose quantitative bioanalytical assays aligned with FDA, EMA, and ICH expectations.
  • Develop and optimize sample extraction strategies (PPT, SPE, LLE, affinity capture) to maximize assay sensitivity, recovery, robustness, and reproducibility.
  • Author method development reports, SOPs, and technical documentation supporting assay transfer, qualification, and validation in compliance with ALCOA+ data integrity standards.
Nov 2024 – May 2025 Food and Drug Administration (FDA/CDER) St. Louis, Missouri
ORISE Fellow
  • Developed high-resolution LC-MS/MS and ion mobility (HRIM-MS, Cyclic IMS-MS) methods for diastereomeric separation and characterization of synthetic oligonucleotides.
  • Conducted impurity profiling, structural elucidation, and degradation product assessment of oligonucleotide drug substances and products.
  • Authored method development reports and technical documentation following FDA quality and data integrity standards; collaborated with regulatory scientists to translate outcomes into practical analytical workflows.
Aug 2020 – Nov 2024 Virginia Commonwealth University Richmond, Virginia
Graduate Assistant (RA & TA) — Dept. of Pharmaceutics
  • Led LC-MS/MS-based quantitative proteomic analysis of macrophages using Orbitrap Fusion Lumos and Q Exactive HFX, identifying key protein markers linked to lung inflammation.
  • Conducted peptide mapping, PTM analysis, and quantitative proteomics; applied bioinformatics and statistical approaches for pathway and genetic analysis.
  • Led projects on radioimmunotherapy for GBM and RNAi-based ADAR gene modulation for cancer immunotherapy, contributing to an NIH R35 grant proposal.
  • Designed “PESC 709: Pharmaceutical Engineering Lab II” course; developed syllabus, graded papers, and delivered 4 lectures for 12 graduate students.
May 2024 – Sep 2024 Food and Drug Administration (FDA/CDER) St. Louis, Missouri
Summer ORISE Fellow
  • Developed and validated a microchip-based CE–MS (ZipChip) method for high-throughput quantitative impurity analysis of synthetic oligonucleotides.
  • Authored SOPs and validation summaries; presented findings to FDA research and regulatory teams emphasizing applicability for routine QC and lot-release testing.
  • Completed FDA 101 training and a 2-month PBPK Modeling workshop (Dr. Raymond Yang).
Dec 2019 – May 2020 University of South Florida Tampa, Florida
Research Assistant — Dept. of Internal Medicine
  • Developed and optimized lipid-based nanocarriers for targeted intranasal delivery of lapatinib and ketoconazole; characterized nanoparticles and conducted in vitro drug release and cell uptake studies.
  • Contributed to “Preclinical efficacy of TN-1008” — anticancer drug screening, PCR, flow cytometry for gene expression, cell viability, and stemness markers.
Jul 2017 – Aug 2018 Radiant Pharmaceuticals Ltd. Tongi, Bangladesh
Development Pharmacist
  • Led formulation development and analytical method development for tablets and oral liquid dosage forms per USP, BP, and ICH guidelines under cGMP & ISO standards.
Education

Academic background

PhD · 2024 · GPA 3.88
Doctor of Philosophy
Pharmaceutical Engineering
Virginia Commonwealth University — Advisor: Dr. Adam Hawkridge
First individual in the nation to earn a PhD in Pharmaceutical Engineering (featured in VCU News)
MS · 2020 · GPA 4.0
Master of Science
Pharmaceutical Nanotechnology
University of South Florida — Advisors: Dr. Subhra & Dr. Shyam Mohapatra
M.Pharm · 2019 · GPA 3.69
Master of Pharmacy
Pharmaceutical Chemistry
University of Dhaka, Bangladesh
B.Pharm · 2017 · GPA 3.68
Bachelor of Pharmacy (5-year Professional)
Pharmacy
University of Dhaka, Bangladesh — WES: 3.8
Publications

Peer-reviewed research

1
Neutrophil elastase targets select proteins on human blood monocyte-derived macrophage cell surfaces.
Ahmed, N. T., Kummarapurugu, A. B., Zheng, S., Bulut, G., Kang, L., Batheja, A., Hawkridge, A., & Voynow, J. A.
International Journal of Molecular Sciences, 2024
Published DOI →
2
Decoding Oligonucleotide Stereochemistry: Unexpected Disconnect Between Individual Phosphorothioate Configuration Ratios and Intact Sequence Diastereomeric Composition.
Islam, M.R., Ahmed, N.T., Yoon, L., Afon, N.D., Zhang, D., Liang, L., Sommers, C., Rodriguez, J.D., Wang, Y., Chia, E-S., Yang, K.
Analytical Chemistry
Under Review
3
Synergistic antibacterial action of AgNP-ampicillin conjugates: Evading β-lactamase degradation in ampicillin-resistant clinical isolates.
Ayubee, M. S., Akter, F., Ahmed, N. T. et al.
PLoS One, 20(9): e0331669, 2025
Published DOI →
4
Chapter 11 — STING Pathway and Modulation for Cancer Immunotherapy.
Su, T., Ahmed, N. T., Zhou, S., Liu, X., & Zhu, G.
Cancer Immunology and Immunotherapy, Academic Press, 2022
Published DOI →
5
Cytotoxic Compounds Derived from Marine Algicolous and Spongicolous Endophytic Fungi: A Review.
Ahmed, N. T., Noor, S., Rahman, M. M., & Mazid, M. A.
Dhaka University Journal of Pharmaceutical Sciences, 20(2), 247–265, 2021
Published DOI →
6
Advances in Translational Nanotechnology: Challenges and Opportunities.
Mohapatra, S. S., Frisina, R. D., Mohapatra, S., ... Ahmed, N. T. et al.
Applied Sciences, 10(14), 4881, 2020
Published DOI →
7
Synthesis and Functional Evaluation of Nanoparticle Conjugated Antibiotics.
Ahmed, N. T. & Mazid, M. A.
New Biotechnology, 44, S95–S96, 2018
Published DOI →
Conference Presentations
2025
Analytical Procedure Development and Validation of High-throughput ZipChip CZE-MS Method for Quantitative Oligonucleotide Analysis
ASMS 2025, Baltimore, MD & FDA Generic Drug Science Day (GDSD) 2025, Silver Spring, MD
2025
Characterization of Phosphorothioate Diastereomers in Synthetic Oligonucleotides Using High-Resolution Ion Mobility-Mass Spectrometry (HRIM-MS)
ASMS 2025, Baltimore, MD & FDA GDSD 2025, Silver Spring, MD
2025
Neutrophil elastase targets select proteins on human blood monocyte derived macrophage cell surfaces
Pediatric Academic Societies Meeting 2025
2019
Lapatinib and Ketoconazole Combination Therapy using Lipid Micelle Nanoparticles for Treatment of Lung Cancer
NanoFlorida 2019
2018
Endophytic Fungal Metabolites Arrest Cell Cycle of Acute Leukemia Cells
IUBMB Conference, Seoul, South Korea (Travel Grant Awardee)
Skills

Technical expertise

Mass Spectrometry Platforms
SCIEX Triple Quad 6500+ Orbitrap Fusion Lumos Q Exactive HF-X Waters Cyclic IMS-QTOF MOBILion-Agilent 6545XT ZipChip CE-MS
Bioanalytical Methods
LC-MS/MS IP-RPLC-MS HILIC-MS CE-MS Ion Mobility-MS HRIM-MS HPLC-UV SDS-PAGE / WB UV-Vis / IR
Sample Preparation
Protein Precipitation SPE Liquid-Liquid Extraction Affinity Capture Hamilton Nimbus Microlab STAR KingFisher
Proteomics & Bioinformatics
Label-free Quantitation SILAC TMT Peptide Mapping PTM Analysis Glycan Profiling GSEA gProfiler Cytoscape
Software & Informatics
SCIEX Analyst / OS Xcalibur Chromeleon MassHunter BioPharma Finder Proteome Discoverer Skyline Byonic SciNote ELN STARLIMS Watson LIMS
Computational & Statistical
R Programming Python JMP / DOE Berkeley Madonna GastroPlus PBPK Modeling
Regulatory & Compliance
GLP / GxP FDA BMV Guidance ALCOA+ ICH Guidelines SOP Authoring Method Validation cGMP / ISO
Laboratory & Cell Biology
Cell Culture ELISA Flow Cytometry qRT-PCR Confocal Microscopy IHC DLS SEM / TEM siRNA / Transfection
Honors & Leadership

Recognition & service

★
Excellence in Pharmaceutical Engineering Award
School of Pharmacy, Virginia Commonwealth University — 2023
★
Excellence in Student Research — Preclinical Efficacy of TN-1008
Florida High Tech Corridor Council Matching Grants Research Program — 2020
★
National Science and Technology Fellowship for Master’s Research
Ministry of Science and Technology, Bangladesh — 2018
✈
Travel Grant Awardee — IUBMB Conference, Seoul, South Korea
International Union of Biochemistry and Molecular Biology — 2018
🏆
Best Poster Award (1st Place)
North South University Pharma Fest — 2018
🏆
Best Poster Award (2nd Place)
ISE SFEC National Conference, Dhaka, Bangladesh
Leadership & Service
Judge — Virginia Junior Academy of Science Research Symposium
VJAS
2023, 2025
Abstract Reviewer — National Biotechnology Conference
American Association of Pharmaceutical Scientists (AAPS)
2025
Founding President — Pharmaceutical Engineering Graduate Student Assoc.
Virginia Commonwealth University
2021–2023
Treasurer — Pharmaceutics Graduate Student Association
Virginia Commonwealth University
2020–2021
Student Advisor — VCU Health Science Library Advisory Committee
Virginia Commonwealth University
2021–2022
Secretary — New Advancements in Nanotechnology Org. (NANO)
University of South Florida
2019–2020
Contact
Let’s connect & collaborate.

Open to research collaborations, speaking invitations, and scientific discussions. Based in Ashland, Ohio.

EMAILnadiatasnimahmed879@gmail.com
PHONE+1 (813) 405-5652
LOCATIONAshland, Ohio, USA
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